Regulatory

Part:BBa_J119131:Design

Designed by: MWSU Genetics Lab Fall 2012 Tues 8:00   Group: Eckdahl Lab   (2013-01-07)


pTac with mutations


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Oligonucleotides.


Source

The oligonucleotides needed to have sticky ends compatible with those produced by BsaI digestion of J100091 so that Golden Gate Assembly could be used.


References